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Molecular Biology
A Guide to the NGS Workflow
NGS Library Preparation and Sample Prep
KAPA Pure Beads
KAPA Pure Beads from Roche
Be the first to write a review!
Citations:
(235)
Supplier Page
Supplier Page from
Roche for
KAPA Pure Beads
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Product Specs
Item
KAPA Pure Beads
Company
Roche
Price
Supplier Page
View Company Product Page
Catalog Number
07983271001
Quantity
5 mL
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Roche
Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road
Indianapolis
,
Indiana
United States
Phone
: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911
Company Profile
Website:
sequencing.roche.com
Citations (235)
(61)
Fecal microbiota transfer between young and aged mice reverses hallmarks of the aging gut, eye, and brain
Microbiome
December 1, 2022
Aimée Parker, Stefano Romano, Rebecca Ansorge, Asmaa Aboelnour, Gwenaelle Le Gall, George M. Savva, Matthew G. Pontifex, Andrea Telatin, David Baker, Emily Jones,
et al.
quantification. The final pool was double-SPRI size selected between 0.5 and 0.7× bead volumes using
KAPA Pure Beads
(
Roche
), quantified on a Qubit 3.0 fluorimeter and run on a
D5000 ScreenTape
system (
Agilent
) using
(62)
Single transcription factor efficiently leads human induced pluripotent stem cells to functional microglia
Inflammation and Regeneration
December 1, 2022
Iki Sonn, Fumiko Honda-Ozaki, Sho Yoshimatsu, Satoru Morimoto, Hirotaka Watanabe, Hideyuki Okano
KAPA
mRNA Capture Kit (KK8440;
Kapa Biosystems
),
KAPA RNA HyperPrep Kit
(KK8542;
Kapa Biosystems
),
KAPA Pure Beads
(KK8543; Kapa Biosystems), and
SeqCap Adapter Kit A
(
Roche
) according to the manufacturer’s instructions.
(63)
Mini-XT, a miniaturized tagmentation-based protocol for efficient sequencing of SARS-CoV-2
Journal of Translational Medicine
December 1, 2022
Marc Fuchs, Clara Radulescu, Miao Tang, Arun Mahesh, Deborah Lavin, Syed Umbreen, James McKenna, Mark Smyth, Eilís McColgan, Zoltan Molnar,
et al.
cleaning was performed using a Biomek NXp robot (Beckman Coulter. CA) with an 8 channel head using 1.5 ×
KAPA Pure beads
(
Roche
) to sample ratio. Samples were eluted in 60 µl 10 mM Tris pH 8.0 and quantified using an Invitrogen
(64)
Heterologous Expression of Extracellular Proteinase pAsPs of Aspergillus pseudotamarii in Komagataella phaffii
International Journal of Molecular Sciences
November 30, 2022
Andrey Valentinovich Zadorozhny, Mikhail Evgenyevich Voskoboev, Denis Vladimirovich Bochkov, Alexei Sergeyevich Rozanov, Elizaveta Dmitrievna Shedko, Irina Anatolyevna Mescheryakova, Alexander Gennadyevich Blinov, Anton Vladimirovich Korzhuk, Valeria Nikolayevna Shlyakhtun, Natalia Vladimirovna Bogacheva,
et al.
alkaline phosphatase was performed at 16 °C for 120 min. After that, the fragments were purified on
KAPA Pure Beads
magnetic particles (
Roche
, Basel, Switzerland) according to the manufacturer’s instructions. Derived
(65)
Mechanism of KIT gene regulation by GATA1 lacking the N-terminal domain in Down syndrome-related myeloid disorders
Scientific reports
November 29, 2022
Kanezaki R, Toki T, Terui K, Sato T, Kobayashi A, Kudo K, Kamio T, Sasaki S, Kawaguchi K, Watanabe K,
et al
.
and H3K4me3 (
9751
,
Cell Signaling Technology
). Libraries were size selected for 150–350 bp range with
KAPA Pure Beads
(
KAPA BIOSYSTEMS
). Samples were sequenced on the Illumina MiSeq (2 × 75 bp). Sequence reads were aligned
(66)
Draft Genome Sequence of Pseudomonas sp. Strains MWU12-2319 and MWU12-2311, Isolated from a Wild Cranberry Bog in the Cape Cod National Seashore
Microbiology Resource Announcements
November 17, 2022
Arin Pittman, Scott Soby
Technologies, Coralville, IA) were ligated to each sample. Adapter-ligated molecules were cleaned using
KAPA pure beads
(
Kapa Biosystems
product number KK8002) and amplified with
KAPA HiFi
enzyme (
Kapa Biosystems
product
(67)
Draft Genome Sequence of Pseudomonas sp. Strain MWU13-3659, Isolated from Commercial Cranberry Bog Soil in Massachusetts, USA
Microbiology Resource Announcements
November 17, 2022
Amritpal Kooner, Scott Soby
Illumina-compatible adapters (product number 00989130v2; Integrated DNA Technologies, Coralville, IA), cleaned using
KAPA pure beads
(
Kapa Biosystems
product number KK8002), and amplified with
KAPA HiFi
enzyme (
Kapa Biosystems
product
(68)
Microbiota Dysbiosis and Gut Barrier Dysfunction Associated with Non-Alcoholic Fatty Liver Disease Are Modulated by a Specific Metabolic Cofactors’ Combination
International Journal of Molecular Sciences
November 8, 2022
Sergio Quesada-Vázquez, Caitlin Bone, Shikha Saha, Iris Triguero, Marina Colom-Pellicer, Gerard Aragonès, Falk Hildebrand, Josep M. del Bas, Antoni Caimari, Naiara Beraza,
et al.
EB (10 mM Tris-HCl), and libraries were performed. Briefly, following a first
PCR
and
clean-up
using
KAPA Pure Beads
(
Roche
Catalogue No.
07983298001
), a second PCR master mix was made up using P7 and P5 of Nextera XT
(69)
Deciphering the impact of genetic variation on human polyadenylation using APARENT2
Genome Biol
November 5, 2022
Johannes Linder, Samantha E. Koplik, Anshul Kundaje, Georg Seelig
\usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\ge$$\end{document}≥ 100nt using
KAPA Pure Beads
(
Roche
).
Sample size
distributions were analyzed using Tapestation High
Sensitivity
D1000 (Agilent). Library
(70)
Acute depletion of human core nucleoporin reveals direct roles in transcription control but dispensability for 3D genome organization
Cell Reports
November 1, 2022
Xiaoyu Zhu, Chuangye Qi, Ruoyu Wang, Joo-Hyung Lee, Jiaofang Shao, Lanxin Bei, Feng Xiong, Phuoc T. Nguyen, Guojie Li, Joanna Krakowiak,
et al.
RNase A
,
Thermo Scientific
,
EN0531
"
Phenol
:Chloroform:Iso-amyl alcohol (125:24:1), pH 4.5",
Thermo Fisher
,
AM9722
KAPA pure beads
,
Roche
/
KAPA
,KK8001 Cyanine 3-
UTP
,
Enzo
Life Science,
ENZ-42505
GlycoBlue™ Coprecipitant,Thermo Fisher,Thermo
(71)
Multiomics analysis reveals that hepatocyte nuclear factor 1β regulates axon guidance genes in the developing mouse kidney
Scientific Reports
October 20, 2022
Annie Shao, Micah D. Gearhart, Siu Chiu Chan, Zhen Miao, Katalin Susztak, Peter Igarashi
(Integrated DNA Technologies) at
1:50
dilution. Ligated samples were purified with 0.8 × volume of
KAPA Pure beads
(
Roche
). To determine the number of
amplification
samples, a 5% test
library amplification
was carried
(72)
Draft Genome of a Member of the Family Chromobacteriaceae Isolated from Anopheles Mosquitoes in West Africa
Microbiology Resource Announcements
October 20, 2022
Keenan Stephens, Edounou Jacques Gnambani, Etienne Bilgo, Abdoulaye Diabate, Scott Soby
catalog number 00989130v2) were individually ligated to each sample. Ligated molecules were cleaned using
Kapa pure beads
and then amplified with HIFI enzyme (
Kapa
Biosciences, KK8002 and KK2502). Each library was sized with
(73)
Draft Genome Sequence of Pseudomonas sp. Strains MWU12-2020 and MWU12-3103b, Isolated from Wild and Cultivated Cranberry Bogs in Massachusetts
Microbiology Resource Announcements
October 20, 2022
Andrea Ormsby, Scott Soby
adapters (IDT number 00989130v2) were ligated to the A-tailed fragments; the library was cleaned using
KAPA pure beads
(KK8002) and then amplified using HiFi enzyme (
KAPA
KK2502). Fragment sizes were determined using an
(74)
The Cynomolgus Macaque Intestinal Mycobiome Is Dominated by the Kazachstania Genus and K. pintolopesii Species
Journal of Fungi
October 8, 2022
Steve A. James, Aimee Parker, Catherine Purse, Andrea Telatin, David Baker, Sandy Holmes, James Durham, Simon G. P. Funnell, Simon R. Carding
also included in each
PCR
run (see Section 2.5). Following ITS1
PCR
, a 0.7× SPRI
purification
using
KAPA Pure Beads
(
Roche
, Wilmington, MA, USA) was performed and the purified DNA was eluted in 20 µL of EB buffer (10
(75)
High-throughput sequencing SELEX for the determination of DNA-binding protein specificities in vitro
STAR Protocols
September 16, 2022
Raphaël Pantier, Kashyap Chhatbar, Grace Alston, Heng Yang Lee, Adrian Bird
the
purification
of SELEX libraries (83 bp). Alternatives: Here, we used
KAPA Pure beads
(
Roche
, Cat#
07983271001
) to
clean-up
high-throughput sequencing
libraries. Alternative reagents can be used, such as AMPure
(76)
Chidamide plus Tyrosine Kinase Inhibitor Remodel the Tumor Immune Microenvironment and Reduce Tumor Progression When Combined with Immune Checkpoint Inhibitor in Naïve and Anti-PD-1 Resistant CT26-Bearing Mice
International Journal of Molecular Sciences
September 14, 2022
Jia-Shiong Chen, Yi-Chien Hsieh, Cheng-Han Chou, Yi-Hong Wu, Mu-Hsuan Yang, Sz-Hao Chu, Ye-Su Chao, Chia-Nan Chen
index codes were added to attribute sequences to each sample.
PCR
products were purified using the
KAPA Pure Beads
system, and library quality was assessed using a Qsep 100 DNA/RNA Analyzer (BiOptic Inc., new Taipei
(77)
Mosquito tagging using DNA-barcoded nanoporous protein microcrystals
PNAS Nexus
September 12, 2022
Julius D Stuart, Daniel A Hartman, Lyndsey I Gray, Alec A Jones, Natalie R Wickenkamp, Christine Hirt, Aya Safira, April R Regas, Therese M Kondash, Margaret L Yates,
et al.
for 30 s, and 1 cycle at 72°C for
1:00
. Following each
amplification
,
PCR
cleanup was performed using
KAPA Pure Beads
(
Roche
). Size selection for the 218 bp barcode library was performed using
Monarch DNA Gel Extraction Kit
(78)
Hybrid metagenome assemblies link carbohydrate structure with function in the human gut microbiome
Communications Biology
September 8, 2022
Anuradha Ravi et al.
equal quantities. The final pool was double-SPRI size selected between 0.5 and 0.7X bead volumes using
KAPA Pure Beads
(
Roche
, Wilmington, US). The final pool was quantified on a Qubit 3.0 instrument and run on a
D5000 ScreenTape
(79)
Transcriptional dynamics of transposable elements in the type I IFN response in Myotis lucifugus cells
Mobile DNA
September 6, 2022
Giulia Irene Maria Pasquesi, Conor J. Kelly, Andrea D. Ordonez, Edward B. Chuong
adapter dimer formation. Adapter-ligated libraries underwent a double-sided 0.8X/1.0X cleanup with
KAPA Pure Beads
(
Kapa Biosystems
#
07983280001
). Purified, adapter-ligated libraries were amplified using the following
(80)
Genome-wide mapping of
N
4
-methylcytosine at single-base resolution by APOBEC3A-mediated deamination sequencing
Chemical Science
August 31, 2022
Jun Xiong et al.
for 15 min using a Hieff
NGS
® Ultima
DNA Ligation
Module (Yeasen) followed by
DNA purification
using
KAPA Pure beads
(
Roche
). The adaptor
ligation
was confirmed by 20%
native PAGE
. The resulting DNA (16 μL) was added
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KAPA Pure Beads from Roche
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